More than 400 people have signed a petition to stop 120 acres of land in Goldenhill becoming a golf course for the second time.
Plans for an 18-hole course to be built on the former 9-hole Goldenhill course, have been strongly rejected by residents.
Eighty residents said they want the area to become a nature reserve.
They attended a public meeting hosted by Sandyford & Goldenhill Residents Association to air their views.
Rachel Horton, from Talke, said: “My fiancé is a golfer and most golfers are already at established clubs such as Alsager or Burslem.
“Another golf club will not be successful.”
The old Goldenhill course, which closed in 2011, is home to a large variety of wildlife and is used by local residents and visitors in the area.
Amy Barley, from Kidsgrove, said: “This is a great place to have a nature walk with our dog and little girl. We have loved seeing the herons and birds of prey.
“The possibilities are endless and this should be an attraction for people of all ages, not just golfers.”
Domenico Schifano, from Stoke-on-Trent, said: “We have less and less wildlife areas as greedy investors take over these areas for profit to their business.
“Don’t allow it to become another failing golf course.”
The area has been used for walking, fishing, running and biking by residents and visitors since the golf club closed.
However, Stoke-on-Trent City Council are backing the plans for the new golf course.
Cllr Jack Brereton said: “I am pleased to say an expression of interest in the land, to become a golf course again, has been made.
“It has also been agreed through a clear direction that it should be used for leisure of golf activity.
“We need to get the land back into use as it will be a good boost for Goldenhill.”
It was confirmed at the Sandyford & Goldenhill Residents Association meeting that it currently costs £30,000 per year to maintain the area.
So far there have been five expressions of interest in the site – all from developers favouring a golf course development.
(Additional reporting by Charlotte Heywood)